ATOPlex Platform
——Targeted Sequencing Expertise, Right by Your Side
Flexible Design, Streamlined Workflow—Efficient and Cost-Effective Targeted Sequencing from Single Genes to 10,000+ Targets, Unlocking Your Full Sequencing Potential
Based on an ultramultiplex PCR approach, it enables efficient enrichment and library preparation for tens of thousands of targets through a two-step enzymatic reaction in a single tube. Leveraging superior PCR primer design and amplification chemistry, the technology delivers exceptional performance with trace samples (e.g., cfDNA, single cells, ancient DNA, FFPE) for high-sensitivity detection.
Custom Design Single site to multi-gene panels.
Rapid Delivery Efficient production, fast turnaround.
Precision Targeting Multiplexed amplification with high-specificity primers.
Broad Application Research, drug development & molecular diagnostics.
ATOPlex, a custom targeted sequencing panel platform, offers both ready-to-use and customized products. These panels are suitable for diverse sample types including blood, cell-free nucleic acids, FFPE samples, and environmental extracts. They are compatible with a wide range of automation equipment and support sequencing on both DNBSEQ™ and CycloneSEQ™ platforms. ATOPlex panels enable the precise detection of various mutations such as SNVs (Single Nucleotide Variants), Indels (Insertions/Deletions), STRs (Short Tandem Repeats), CNVs (Copy Number Variations), and Fusions, comprehensively meeting application requirements across agriculture, public health, environmental monitoring, food safety, and other fields.
Ready-to-Use Panels
Pre-configured targeted sequencing products for universal applications. Covers public health, environmental testing, agricultural breeding, and forensics.
Custom Panels
Tailored panel developed for your specific needs. Submit region of interest via . Production and delivery within 4 weeks upon agreement finalization.
ATOPlex technology
Based on an ultramultiplex PCR approach, it enables efficient enrichment and library preparation for tens of thousands of targets through a two-step enzymatic reaction in a single tube. Leveraging superior PCR primer design and amplification chemistry, the technology delivers exceptional performance with trace samples (e.g., cfDNA, single cells, ancient DNA, FFPE) for high-sensitivity detection.
Auto-workflow Automated Design & Operation
- Design automation
- Process automation
Trace-detection Minimal Input with Crude Sample
- Ultra-low sample requirement
- Direct amplification
One-tube Single-tube Processing
- Ultrahigh-plex in single tube
- No tube changes
Pure-PCR Superior Contaminant Resistance
- Inhibitor tolerance
- Aerosol contamination prevention
Amplification strategy
The ATOPlex technology employs a two-step PCR approach to achieve target region enrichment and sample barcoding.
ATOPlex 2-Step strategy
Application case
ATOPlex employs ultra-multiplex PCR technology to achieve high-specificity enrichment with minimal dimer formation. The right figure demonstrates the enrichment performance of ATOPlex technology—designed with ultrashort amplicons (60-90 bp) targeting 2,009 high-frequency SNP loci (population frequency >0.3)—on trace nucleic acid samples. Results show excellent sequencing performance of ATOPlex libraries on the DNBSEQ platform.
Figure1: Agilent 2100 bioanalyzer reveals that this is no non-specific products and slightly primer dimer in the high-plex amplicon library
Figure2: The sequencing depth of each amplicon with different GC contents at a mean sequencing depth of 1000X
Figure3: The sequencing depth of each amplicon in two separate experiments
Total solution
MGI develops streamlined omics tools for life sciences, delivering simplicity, speed, and precision. Offering end-to-end targeted sequencing solutions—from sample prep and library construction to high-throughput sequencing and bioinformatic analysis—tailored for your laboratory.
