ATOPlex Platform
——Targeted Sequencing Expertise, Right by Your Side
Flexible Design, Streamlined Workflow—Efficient and Cost-Effective Targeted Sequencing from Single Genes to 10,000+ Targets, Unlocking Your Full Sequencing Potential
Based on an ultramultiplex PCR approach, it enables efficient enrichment and library preparation for tens of thousands of targets through a two-step enzymatic reaction in a single tube. Leveraging superior PCR primer design and amplification chemistry, the technology delivers exceptional performance with trace samples (e.g., cfDNA, single cells, ancient DNA, FFPE) for high-sensitivity detection.
Based on an ultramultiplex PCR approach, it enables efficient enrichment and library preparation for tens of thousands of targets through a two-step enzymatic reaction in a single tube. Leveraging superior PCR primer design and amplification chemistry, the technology delivers exceptional performance with trace samples (e.g., cfDNA, single cells, ancient DNA, FFPE) for high-sensitivity detection.
ATOPlex 2-Step strategy
Figure1: Agilent 2100 bioanalyzer reveals that this is no non-specific products and slightly primer dimer in the high-plex amplicon library
Figure2: The sequencing depth of each amplicon with different GC contents at a mean sequencing depth of 1000X
Figure3: The sequencing depth of each amplicon in two separate experiments